Aimed at the problem of excessive vibration noise of urban substation reactor chamber, the comprehensive reform of the project was carried out by using vibration and noise reduction technology. Reasonable isolation device based on the principle of reactor vibration model was designed, as well as typical position of vibration isolation scheme in real time was detected with using the online vibration transfer function and vibration spectrum. The transmission route of noise could be controlled by using the noise reduction measures of sound absorption, sound insulation, noise elimination, etc. Meanwhile, the noise data of indoor and outdoor reactor chamber was acquired and analyzed before and after transformation. The results show that the effect of vibration isolation scheme using the damping pad and the six vibration absorbers at the bottom is good, in which the isolation rate of body is more than 93%. Through using comprehensive measures, such as the reasonable selection of porous fiber acoustic materials, the transformation of air inlet and soundproof door, the applications of low noise fan and muffler, etc., the noise inside the reactor chamber can be reduced to about 8 dB, and the outdoor noise is equal to environmental noise, which shows that the noise control achieves the desired effect. The successful implementation of this research can provide technical support for the future project transformation.